Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli | Zendy

Wee Liang Kuan | Zendy; Joma Joy | Zendy; Ng Fui Mee | Zendy; Kwek Zekui Perlyn | Zendy; Then Siew Wen | Zendy; Thuy Nguen | Zendy; Joanne James | Zendy; Elaine Chai | Zendy; Horst Flotow | Zendy; Sharon Crasta | Zendy; Kian Ngiap Chua | Zendy; Ng Sok Peng | Zendy; Jeffrey Hill | Zendy

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Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli

Author(s) -

Wee Liang Kuan,

Joma Joy,

Ng Fui Mee,

Kwek Zekui Perlyn,

Then Siew Wen,

Thuy Nguen,

Joanne James,

Elaine Chai,

Horst Flotow,

Sharon Crasta,

Kian Ngiap Chua,

Ng Sok Peng,

Jeffrey Hill

Publication year - 2010

Publication title -

biochemistry insights

Language(s) - English

Resource type - Journals

ISSN - 1178-6264

DOI - 10.4137/bci.s5123

Subject(s) - terminal deoxynucleotidyl transferase , recombinant dna , microbiology and biotechnology , tunel assay , escherichia coli , biology , transferase , expression vector , apoptosis , chemistry , enzyme , gene , biochemistry

A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression of active recombinant bovine TdT in E.coli. After purification and characterisation, the activity of the enzyme was assessed in a biological assay for apoptosis. The process described in this report enables the economical production of TdT for high throughput applications

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