Open AccessFast and Simple microRNA Northern Blots
Author(s) -
Nham Tran
Publication year - 2009
Publication title -
biochemistry insights
Language(s) - English
Resource type - Journals
ISSN - 1178-6264
DOI - 10.4137/bci.s2257
Subject(s) - northern blot , blot , microrna , southern blot , microbiology and biotechnology , simple (philosophy) , biology , gene expression , computational biology , gene , genetics , philosophy , epistemology
RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours
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