Determination and Validation of a Solid-phase Extraction Gas Chromatography-mass Spectrometry for the Quantification of Methadone and Its Principal Metabolite in Human Plasma | Zendy

Fouad Chiadmi | Zendy; Joël Schlatter | Zendy

Open Access

Determination and Validation of a Solid-phase Extraction Gas Chromatography-mass Spectrometry for the Quantification of Methadone and Its Principal Metabolite in Human Plasma

Author(s) -

Fouad Chiadmi,

Joël Schlatter

Publication year - 2015

Publication title -

analytical chemistry insights

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.406

H-Index - 17

ISSN - 1177-3901

DOI - 10.4137/aci.s25554

Subject(s) - chromatography , metabolite , mass spectrometry , gas chromatography–mass spectrometry , solid phase extraction , analyte , gas chromatography , pharmacokinetics , extraction (chemistry) , methadone , therapeutic drug monitoring , selected ion monitoring , detection limit , medicine , chemistry , pharmacology

This study aimed to develop a solid-phase extraction gas chromatography-selected ion monitoring-mass spectrometry method for the determination of methadone (MDN) and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human plasma. The linear response was obtained over the concentration range from 10 to 2000 ng/mL for MDN and EDDP. The absolute recoveries of MDN and EDDP were 95.9%-98.9% and 94.8%-102.4%, with relative standard deviation (RSD) ranging from 1.8% to 2.7% and 1.8% to 3.9%, respectively. The intra- and interday precisions were found to be less than 5% for both analytes. The limits of detection of MDN and EDDP were 4 and 5 ng/mL, respectively. The presented method was convenient for therapeutic drug monitoring and pharmacokinetic studies in patients on heroin-assisted MDN therapy.

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