Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells | Zendy

Hamid Galehdari | Zendy; Samira Negahdari | Zendy; Mahnaz Kesmati | Zendy; Annahita Rezaie | Zendy; Gholamreza Shariati | Zendy

Open Access

Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells

Author(s) -

Hamid Galehdari,

Samira Negahdari,

Mahnaz Kesmati,

Annahita Rezaie,

Gholamreza Shariati

Publication year - 2017

Publication title -

international journal of preventive medicine

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.539

H-Index - 39

eISSN - 2008-8213

pISSN - 2008-7802

DOI - 10.4103/ijpvm.ijpvm_338_16

Subject(s) - aloe vera , fibroblast , wound healing , dermal fibroblast , transforming growth factor , gene expression , in vitro , traditional medicine , vascular endothelial growth factor , foreskin , gene , microbiology and biotechnology , biology , pharmacology , medicine , vegf receptors , immunology , cell culture , cancer research , biochemistry , genetics

Background: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts.Methods: Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgf β 1and Vegf-A genes were monitored by real-time polymerase chain reaction.Results: A. capillus-veneris, C. molmol and henna extract improved the expression of Tgf β 1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro.Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts

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