Extraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears | Zendy

Ravindra R. Kamble | Zendy; Shinde Vs | Zendy; SP Madhale | Zendy; AA Kamble | Zendy; BP Ravikumar | Zendy; R S Jadhav | Zendy

Open Access

Extraction and detection of Mycobacterium leprae DNA from ZNCF-stained skin smear slides for better identification of negative skin smears

Author(s) -

Ravindra R. Kamble,

Shinde Vs,

SP Madhale,

AA Kamble,

BP Ravikumar,

R S Jadhav

Publication year - 2010

Publication title -

indian journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.382

H-Index - 48

eISSN - 1998-3646

pISSN - 0255-0857

DOI - 10.4103/0255-0857.58732

Subject(s) - mycobacterium leprae , leprosy , dna extraction , proteinase k , staining , stain , biology , microbiology and biotechnology , pathology , polymerase chain reaction , dna , medicine , biochemistry , genetics , gene

Abstract Identification of Mycobacterium leprae, which causes leprosy, is done by Ziehl Neelsen Carbol Fuchsin (ZNCF) stained slit skin smear microscopy that aids in the diagnosis and quantification of approximate bacterial load carried by the patient. We attempted M. leprae DNA extraction from 46 stained slit skin smear negative slides, using Proteinase K and SDS lysis, followed by ethanol precipitation. M. leprae specific primers (16SrRNA) were used for PCR-based amplification of DNA. We could detect M. leprae DNA in 15 (32.6%) samples. The method can be useful in the diagnosis of apparently slit skin smear negative leprosy cases.

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