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Experiments were performed with the aim of investigating the effect of packing on erythrocyte  osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the  effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional  extensive system served as experimental subjects. Six donkeys administered orally with AA  (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not  administered with AA served as controls. Blood samples were collected pre- and post-packing  from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl)  concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the  value was significantly (P &amp;lt; 0.05) higher than the value of 71.31% ± 8.33%, recorded in the  experimental donkeys. The post-packing MDA concentration obtained in the control donkeys  was 39.62 µmol ± 4.16 µmol, and was not significantly different (P &amp;gt; 0.05) from the value of  35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in  haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which  was ameliorated by AA administration.

Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid