Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23 | Zendy

Tawin Inpankaew | Zendy; Sathaporn Jittapalapong | Zendy; Jumgjit Phasuk | Zendy; N. Pinyopanuwut | Zendy; W. Chimnoi | Zendy; C. Kengradomkit | Zendy; Chainirun Sunanta | Zendy; G. ZHANG | Zendy; Gabriel Oluga Aboge | Zendy; Yoshifumi Nishikawa | Zendy; Ikuo Igarashi | Zendy; Xuenan Xuan | Zendy

Open Access

Seroprevalence of <i>Cryptosporidium parvum</i> infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23

Author(s) -

Tawin Inpankaew,

Sathaporn Jittapalapong,

Jumgjit Phasuk,

N. Pinyopanuwut,

W. Chimnoi,

C. Kengradomkit,

Chainirun Sunanta,

G. ZHANG,

Gabriel Oluga Aboge,

Yoshifumi Nishikawa,

Ikuo Igarashi,

Xuenan Xuan

Publication year - 2009

Publication title -

onderstepoort journal of veterinary research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.563

H-Index - 35

eISSN - 2219-0635

pISSN - 0030-2465

DOI - 10.4102/ojvr.v76i2.40

Subject(s) - cryptosporidium parvum , seroprevalence , veterinary medicine , outbreak , cryptosporidium , chiang mai , virology , population , antigen , biology , serology , feces , medicine , antibody , microbiology and biotechnology , immunology , environmental health , history , ethnology

Cryptosporidium parvum is the most frequent parasitic agent that causes diarrhoea in AIDS patients in Thailand. Cryptosporidiosis outbreaks in humans may be attributed to contamination of their drinking water from infected dairy pastures. A 23-kDa glycoprotein of C. parvum (CpP23) is a sporozoite surface protein that is geographically conserved among C. parvum isolates. This glycoprotein is a potentially useful candidate antigen for the diagnosis of cryptosporidiosis by enzyme-linked immunosorbent assay. Therefore, we investigated the seroprevalence of C. parvum infection in dairy cows in northern Thailand using an ELISA based on recombinant CpP23 antigen. Sera were randomly collected from 642 dairy cows of 42 small-holder farmers, which had the top three highest number of the dairy cows' population in Northern Thailand, that included Chiang Mai, Chiang Rai and Lumpang provinces. The overall seroprevalence of the infection was 4.4%, and the seropositive rates for the three provinces were 3.3% in Chiang Mai, 5.1% in Chiang Rai and 3% in Lumpang. These results suggest that cattle could play a role in zoonotic cryptosporidiosis in Thailand.

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