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Rhodamine B is a textile dye compounds containing chlorine (Cl-), alkylating (CH3-CH3), Poly Aromatic Hydrocarbons (PAH) which activate the enzyme cytochrome P-450 as well as the structure of quinone which is very redox that leads to the formation of Reactive Oxygen Species (ROS). ROS increases induce apoptosis of the intrinsic pathway. The imbalance ratio between BAX and BCL-2 stimulates apoptosis in Hippocampus tissue. “The selected design was “the post test only control group” using twenty-eight Wistar female Rattus norvegicus mouse age of 10-12 weeks. There was a significant difference (p-value <0.05) of total BCL-2 expression between the control group to the treatment group. Correlation coefficient of 0.945 indicates that the level of the relationship/ correlation is very strong category. Increasing doses of Rhodamine B was given, accompanied by the decrease in the expression of BCL-2. Correlation coefficient of -0.731 indicates that the level of the relationship/ correlation belongs strong category. It is concluded that Rhodamin B has been verified as capable to increase the expression of BAX and to reduce the expression of BCL-2 in hippocampus tissue on Rattus norvegicus.

journal of public health in africa

Rhodamin-B increases Hippocampus cell apoptosis in Rattus norvegicus-oxidative stress related to Parkinson, Alzheimer, cancer, hyperactive, anterograde amnesia diseases