Open AccessEvaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy
Author(s) -
Elisa Massella,
Silvia Piva,
Federica Giacometti,
G. Liuzzo,
Angelo Vittorio Zambrini,
Andrea Serraino
Publication year - 2017
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2017.6904
Subject(s) - casein , bovine milk , genotype , raw milk , beta (programming language) , population , polymorphism (computer science) , food science , exon , biology , gene , zoology , microbiology and biotechnology , genetics , medicine , environmental health , computer science , programming language
Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2- milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis. In the population included in the study 5 variants (A1, A2, B, F, I) and 13 genotypes (A1A1, A1A2, A1B, A1F, A1I, A2A2, A2B, A2F, A2I, BB, BF, BI, FI) were detected. The method showed high sensibility and specificity, resulted low-cost and few time consuming
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