Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium | Zendy

Andrea Ferrigno | Zendy; Laura Giuseppina Di Pasqua | Zendy; Clarissa Berardo | Zendy; Veronica Siciliano | Zendy; Plinio Richelmi | Zendy; Mariapia Vairetti | Zendy

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Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium

Author(s) -

Andrea Ferrigno,

Laura Giuseppina Di Pasqua,

Clarissa Berardo,

Veronica Siciliano,

Plinio Richelmi,

Mariapia Vairetti

Publication year - 2017

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2017.2798

Subject(s) - trypan blue , chemistry , hypoxia (environmental) , sodium , tbars , staining , oxygen , microbiology and biotechnology , biochemistry , cell , biology , pathology , lipid peroxidation , medicine , oxidative stress , organic chemistry

The role of Na⁺ in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N₂-saturated buffer containing standard (143 mM) or low (25 mM) Na⁺ levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na⁺, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na⁺ buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na⁺ buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na⁺ buffer. Using low-Na⁺ buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.