Expression of the HPV18/E6 oncoprotein induces DNA damage | Zendy

Elva I. CortésGutiérrez | Zendy; Catalina Garcí­a-Vielma | Zendy; Adriana AguilarLemarroy | Zendy; Verónica VallejoRuiz | Zendy; Patricia PiñaSánchez | Zendy; Pablo ZapataBenavides | Zendy; Jaime Gosálvez | Zendy

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Expression of the HPV18/E6 oncoprotein induces DNA damage

Author(s) -

Elva I. CortésGutiérrez,

Catalina García-Vielma,

Adriana AguilarLemarroy,

Verónica VallejoRuiz,

Patricia PiñaSánchez,

Pablo ZapataBenavides,

Jaime Gosálvez

Publication year - 2017

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2017.2773

Subject(s) - oncogene , hela , dna , microbiology and biotechnology , dna damage , carcinogenesis , in situ hybridization , biology , comet assay , chemistry , gene expression , cell cycle , gene , genetics , cell culture

This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the HPV/E6 oncogene compared with HeLa cells with normal expression of the E6 oncogene using the DNA breakage detection–fluorescence in situ hybridization (DBD–FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the HPV18/E6 oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the E6 oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the HPV/E6 oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.