Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation | Zendy

Mariko Kawai | Zendy; Yu-Ki Ohmori | Zendy; Mai Nishino | Zendy; Masayo Yoshida | Zendy; Kaori Tabata | Zendy; Do-Saku Hirota | Zendy; Ayako Ryu-Mon | Zendy; Hiromitsu Yamamoto | Zendy; Junya Sonobe | Zendy; Yo-Hei Kataoka | Zendy; Noriko Shiotsu | Zendy; Mika Ikegame | Zendy; Hiroki Maruyama | Zendy; Toshio Yamamoto | Zendy; Kazuhisa Bessho | Zendy; Kiyoshi Ohura | Zendy

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Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

Author(s) -

Mariko Kawai,

Yu-Ki Ohmori,

Mai Nishino,

Masayo Yoshida,

Kaori Tabata,

Do-Saku Hirota,

Ayako Ryu-Mon,

Hiromitsu Yamamoto,

Junya Sonobe,

Yo-Hei Kataoka,

Noriko Shiotsu,

Mika Ikegame,

Hiroki Maruyama,

Toshio Yamamoto,

Kazuhisa Bessho,

Kiyoshi Ohura

Publication year - 2017

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2017.2772

Subject(s) - electroporation , microbiology and biotechnology , stem cell , skeletal muscle , bone morphogenetic protein 2 , bone morphogenetic protein , biology , regeneration (biology) , bone morphogenetic protein 6 , adult stem cell , bone morphogenetic protein 7 , cellular differentiation , anatomy , in vitro , gene , genetics

We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regeneration-related markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer.