3-hydroxi-anthranilic acid is early expressed in stroke | Zendy

A. Mangas | Zendy; Javier Yajeya | Zendy; N. González | Zendy; I. Ruiz | Zendy; M. Geffard | Zendy; Rafael Coveñas | Zendy

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3-hydroxi-anthranilic acid is early expressed in stroke

Author(s) -

A. Mangas,

Javier Yajeya,

N. González,

I. Ruiz,

M. Geffard,

Rafael Coveñas

Publication year - 2016

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2016.2709

Subject(s) - immunohistochemistry , stroke (engine) , pathology , cerebral cortex , striatum , monoclonal antibody , medicine , astrocyte , anthranilic acid , microglia , distribution (mathematics) , cortical spreading depression , chemistry , antibody , central nervous system , immunology , biochemistry , inflammation , mechanical engineering , mathematical analysis , mathematics , migraine , engineering , dopamine

Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.

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