Seasonal changes of androgen receptor, estrogen receptors and aromatase expression in the medial preoptic area of the wild male ground squirrels (Citellus dauricus Brandt) | Zendy

Fengwei Zhang | Zendy; Jianshe Wang | Zendy; Yuanyuan Jiao | Zendy; Liqiu Zhang | Zendy; H. Zhang | Zendy; Xia Sheng | Zendy; Yingying Han | Zendy; Zhengrong Yuan | Zendy; Qihao Weng | Zendy

Open Access

Seasonal changes of androgen receptor, estrogen receptors and aromatase expression in the medial preoptic area of the wild male ground squirrels (Citellus dauricus Brandt)

Author(s) -

Fengwei Zhang,

Jianshe Wang,

Yuanyuan Jiao,

Liqiu Zhang,

H. Zhang,

Xia Sheng,

Yingying Han,

Zhengrong Yuan,

Qihao Weng

Publication year - 2016

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2016.2621

Subject(s) - medicine , endocrinology , androgen receptor , estrogen receptor , hibernation (computing) , hypothalamus , seasonal breeder , androgen , biology , aromatase , preoptic area , estrogen , ground squirrel , arvicolinae , hormone , ecology , prostate cancer , thermoregulation , cancer , breast cancer , state (computer science) , algorithm , microtus , computer science

The wild ground squirrel is a typical seasonal breeder. In this study, using RT-PCR, western blot and immunohistochemistry, we investigated the mRNA and protein expressions of androgen receptor (AR), estrogen receptors α and β (ERα and ERβ) and aromatase cytochrome P450 (P450arom) in the medial preoptic area (MPOA) of hypothalamus of the wild male ground squirrel during the breeding season (April), the non-breeding season (June) and pre-hibernation (September). AR, ERα, ERβ and P450arom protein/mRNA were present in the MPOA of all seasons detected. The immunostaining of AR and ERα showed no significant changes in different periods, whereas ERβ and P450arom had higher immunoreactivities during the breeding season and pre-hibernation when compared to those of the non-breeding season. Consistently, both the protein and mRNA levels of P450arom and ERβ were higher in the MPOA of pre-hibernation and the breeding season than in the non-breeding season, whereas no significant difference amongst the three periods was observed for AR and ERα levels. These findings suggested that the MPOA of hypothalamus may be a direct target of androgen and estrogen. Androgen may play important regulatory roles through its receptor and/or the aromatized estrogen in the MPOA of hypothalamus of the wild male ground squirrels.

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