Analysis of ERK3 intracellular localization: dynamic distribution during mitosis and apoptosis | Zendy

Francesca Aredia | Zendy; Manuela Malatesta | Zendy; Paola Veneroni | Zendy; Maria Grazia Bottone | Zendy

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Analysis of ERK3 intracellular localization: dynamic distribution during mitosis and apoptosis

Author(s) -

Francesca Aredia,

Manuela Malatesta,

Paola Veneroni,

Maria Grazia Bottone

Publication year - 2015

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2015.2571

Subject(s) - nucleoplasm , microbiology and biotechnology , apoptosis , cell cycle , mitosis , immunoelectron microscopy , cytoplasm , mapk/erk pathway , interphase , hela , intracellular , kinase , biology , cell nucleus , chemistry , cell , nucleolus , immunology , biochemistry , antibody

Extracellular signal-regulated kinases (ERK) 1, 2 and 3 are involved in cell proliferation and differentiation, and apoptosis; although ERK1/2 have been widely studied, limited knowledge on ERK3 is available. The present work aimed at investigating ERK3 distribution during cell cycle and apoptosis in human tumor HeLa cells. The analysis performed by double immunofluorescence and immunoelectron microscopy experiments revealed that during interphase ERK3 is mainly resident in the nucleoplasm in association with ribonuclear proteins involved in early pre-mRNA splicing, it undergoes cell cycle-dependent redistribution and, during apoptosis, it remains in the nucleus in the form of massive nuclear aggregates, then moves to the cytoplasm and is finally extruded.

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