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Diaminobenzidine photoconversion is a  technique by which a fluorescent dye is transformed  into a stably insoluble, brown, electrondense  signal, thus enabling examination at  both bright field light microscopy and transmission  electron microscopy. In this work, a  procedure is proposed for combining photoconversion  and immunoelectron microscopy:  <em>in vitro</em> cell cultures have been first submitted  to photoconversion to analyse the intracellular  fate of either fluorescent nanoparticles or photosensitizing  molecules, then processed for  transmission electron microscopy; different  fixative solutions and embedding media have  been used, and the ultrathin sections were  finally submitted to post-embedding immunogold  cytochemistry. Under all conditions the  photoconversion reaction product and the target  antigen were properly detected in the same  section; Epon-embedded, osmicated samples  required a pre-treatment with sodium metaperiodate  to unmask the antigenic sites. This  simple and reliable procedure exploits a single  sample to simultaneously localise the photoconversion  product and a variety of antigens  allowing a specific identification of subcellular  organelles at the ultrastructural level

Simultaneous ultrastructural analysis of fluorochrome-photoconverted diaminobenzidine and gold immunolabeling in cultured cells