In vitro reparative dentin: a biochemical and morphological study | Zendy

Gabriella Teti | Zendy; Viviana Salvatore | Zendy; Alessandra Ruggeri | Zendy; Lucia Manzoli | Zendy; Marco Gesi | Zendy; Giovanna Orsini | Zendy; Mirella Falconi | Zendy

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In vitro reparative dentin: a biochemical and morphological study

Author(s) -

Gabriella Teti,

Viviana Salvatore,

Alessandra Ruggeri,

Lucia Manzoli,

Marco Gesi,

Giovanna Orsini,

Mirella Falconi

Publication year - 2013

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2013.e23

Subject(s) - odontoblast , dmp1 , dentinogenesis , extracellular matrix , dentin , chemistry , pulp (tooth) , microbiology and biotechnology , type i collagen , dentin sialophosphoprotein , pathology , biology , biochemistry , viral matrix protein , medicine , gene

In this study, starting from human dental pulp cells cultured in vitro, we simulated reparative dentinogenesis using a medium supplemented with different odontogenic inductors. The differentiation of dental pulp cells in odontoblast-like cells was evaluated by means of staining, and ultramorphological, biochemical and biomolecular methods. Alizarin red staining showed mineral deposition while transmission electron microscopy revealed a synthesis of extracellular matrix fibers during the differentiation process. Biochemical assays demonstrated that the differentiated phenotype expressed odontoblast markers, such as Dentin Matrix Protein 1 (DMP1) and Dentin Sialoprotein (DSP), as well as type I collagen. Quantitative data regarding the mRNA expression of DMP1, DSP and type I collagen were obtained by Real Time PCR. Immunofluorescence data demonstrated the various localizations of DSP and DMP1 during odontoblast differentiation. Based on our results, we obtained odontoblast-like cells which simulated the reparative dentin processes in order to better investigate the mechanism of odontoblast differentiation, and dentin extracellular matrix deposition and mineralization.

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