NADPH-d activity in rat thymus after the application of retinoid acid | Zendy

F. Dorko | Zendy; Tatiana Špakovská | Zendy; Květuše Lovásová | Zendy; Peter Patlevič | Zendy; D Kluchová | Zendy

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NADPH-d activity in rat thymus after the application of retinoid acid

Author(s) -

F. Dorko,

Tatiana Špakovská,

Květuše Lovásová,

Peter Patlevič,

D Kluchová

Publication year - 2012

Publication title -

european journal of histochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.754

H-Index - 42

eISSN - 2038-8306

pISSN - 1121-760X

DOI - 10.4081/ejh.2012.e7

Subject(s) - nitric oxide synthase , nadph dehydrogenase , nitric oxide , retinoid , endocrinology , medicine , nicotinamide adenine dinucleotide phosphate , nicotinamide , gestation , diaphorase , chemistry , biology , retinoic acid , anatomy , biochemistry , enzyme , gene , pregnancy , genetics , oxidase test

The aim of this work was to determine the localization of nicotinamide-adenine dinucleotide phosphate-diaphorase (NADPH-d) activity as the marker for synthesis of nitric oxide synthase (NOS) in the rat thymus after the application of retinoid acid (RA) on 1st, 7th, 14th and 21st days of gestation. The given results can build the basis for understanding of the role of NOS in rat thymus. NADPH-d positive cells were represented with dark-blue color and were localized on corticomedullar junction of the thymus. These cells were of different intensity of coloring and were shaped in oval, circle or irregular forms. NADPH-d positive nerve fibers were observed in perivascular topography. They were marked more strongly in the case of control group. The result of application of RA to gravid rats was that the birth weights of newborn rats and their thymuses were smaller, but without statistically significance

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