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Objective: To investigate the effect of tumor necrosis factor (TNF)-α on the production of macrophage migration inhibitory factor (MIF), which might have important roles in the immune mediated inflammatory response of Behcet's syndrome. Methods: Sixty two patients with Behcet's syndrome and thirty healthy controls were included in this study. The concentrations of TNF-α in sera were determined by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) from eleven patients with Behcet's syndrome were cultured for 48 hours with various concentration of TNF-α. The concentrations of MIF in sera and culture supernatants were determined by ELISA. Results: Serum levels of TNF-α were significantly higher in patients with Behcet's syndrome than in healthy controls. TNF-α dose-dependently increased MIF production from PBMCs in

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Upregulation of Macrophage Migration Inhibitory Factor (MIF) Production from Peripheral Blood Mononuclear Cells (PBMCs) Stimulated by Tumor Necrosis Factor (TNF)-α in Patients with Behcet's Syndrome