Establishment of Methylation-Specific PCR for the Mouse p53 Gene | Zendy

Ryuji Okazaki | Zendy; Akira Ootsuyama | Zendy; Yasuhiro Yoshida | Zendy; Toshiyuki Norimura | Zendy

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Establishment of Methylation-Specific PCR for the Mouse p53 Gene

Author(s) -

Ryuji Okazaki,

Akira Ootsuyama,

Yasuhiro Yoshida,

Toshiyuki Norimura

Publication year - 2011

Publication title -

molecular biology international

Language(s) - English

Resource type - Journals

eISSN - 2090-2190

pISSN - 2090-2182

DOI - 10.4061/2011/938435

Subject(s) - primer (cosmetics) , dna methylation , microbiology and biotechnology , methylation , cpg site , gene , epigenetics , dna , bisulfite sequencing , polymerase chain reaction , biology , illumina methylation assay , genetics , gene expression , chemistry , organic chemistry

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with both primers. In radiation-induced tumors, DNA from an old mouse yielded similar bands with both types of primers. We suggest that MSP is a valuable technique for the epigenetic study of the mouse p53 gene.

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