Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification | Zendy

Aaron Burrell | Zendy; Chester L. Foy | Zendy; Malcolm Burns | Zendy

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Applicability of Three Alternative Instruments for Food Authenticity Analysis:GMO Identification

Author(s) -

Aaron Burrell,

Chester L. Foy,

Malcolm Burns

Publication year - 2011

Publication title -

biotechnology research international

Language(s) - English

Resource type - Journals

eISSN - 2090-3138

pISSN - 2090-3146

DOI - 10.4061/2011/838232

Subject(s) - capillary electrophoresis , protocol (science) , polymerase chain reaction , identification (biology) , microbiology and biotechnology , genetically modified organism , food science , computational biology , medicine , biology , genetics , gene , pathology , alternative medicine , botany

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients

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