A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody | Zendy

Amanda S. Lakamp | Zendy; Michel M. Ouellette | Zendy

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A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody

Author(s) -

Amanda S. Lakamp,

Michel M. Ouellette

Publication year - 2011

Publication title -

journal of nucleic acids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.621

H-Index - 32

eISSN - 2090-021X

pISSN - 2090-0201

DOI - 10.4061/2011/720798

Subject(s) - aptamer , systematic evolution of ligands by exponential enrichment , antibody , flag (linear algebra) , computational biology , chemistry , dna , microbiology and biotechnology , medicine , biology , immunology , biochemistry , rna , gene , mathematics , pure mathematics , algebra over a field

Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. The aptamer consisted of two motifs (CCTTA and TGTCTWCC) separated by 2-3 bases, and the elimination of one or the other motif abrogated binding. The DNA aptamer and FLAG peptide competed for binding to the antigen-binding pocket of the M2 antibody. In addition, the aptamer eluted FLAG-tagged proteins from the antibody, suggesting a commercial application in protein purification. These findings demonstrate the feasibility of using SELEX to develop ssDNA aptamers that block the function of a specific antibody, a capability that could lead to the development of novel therapeutic modalities for patients with systemic lupus erythematosus, rheumatoid arthritis, and other autoimmune diseases

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