Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH | Zendy

Evelyn Rabelo Andrade | Zendy; P. MaddoxHyttel | Zendy; Fernanda da Cruz LandimAlvarenga | Zendy; J.R.V. Silva | Zendy; Amauri Alcindo Alfieri | Zendy; Marcelo Marcondes Seneda | Zendy; J.R. Figueiredo | Zendy; Ricardo Toniolli | Zendy

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Ultrastructure of Sheep Primordial Follicles Cultured in the Presence of Indol Acetic Acid, EGF, and FSH

Author(s) -

Evelyn Rabelo Andrade,

P. MaddoxHyttel,

Fernanda da Cruz LandimAlvarenga,

J.R.V. Silva,

Amauri Alcindo Alfieri,

Marcelo Marcondes Seneda,

J.R. Figueiredo,

Ricardo Toniolli

Publication year - 2010

Publication title -

veterinary medicine international

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.62

H-Index - 30

eISSN - 2090-8113

pISSN - 2042-0048

DOI - 10.4061/2011/670987

Subject(s) - ultrastructure , cytoplasm , endoplasmic reticulum , epidermal growth factor , lipid droplet , microbiology and biotechnology , oocyte , in vitro , chemistry , mitochondrion , biology , endocrinology , andrology , medicine , cell culture , biochemistry , anatomy , genetics , embryo

The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6 d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro

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