Open AccessKeratinase Production by ThreeBacillusspp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation
Author(s) -
Ana Maria Mazotto,
R. R. R. Coelho,
Sabrina Martins Lage Cedrola,
Marcos Fábio de Lima,
Sônia Couri,
Edilma Paraguai de Souza,
Alane Beatriz Vermelho
Publication year - 2011
Publication title -
enzyme research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.439
H-Index - 39
eISSN - 2090-0406
pISSN - 2090-0414
DOI - 10.4061/2011/523780
Subject(s) - keratinase , feather meal , feather , bacillus licheniformis , bacillus subtilis , biology , food science , fermentation , microbiology and biotechnology , strain (injury) , bacteria , veterinary medicine , fish meal , ecology , medicine , fishery , fish <actinopterygii> , anatomy , genetics
Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity
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