On-Chip DNA Methylation Analysis Using Osmium Complexation | Zendy

Kaori Sugizaki | Zendy; T. Umemoto | Zendy; Akimitsu Okamoto | Zendy

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On-Chip DNA Methylation Analysis Using Osmium Complexation

Author(s) -

Kaori Sugizaki,

T. Umemoto,

Akimitsu Okamoto

Publication year - 2011

Publication title -

journal of nucleic acids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.621

H-Index - 32

eISSN - 2090-021X

pISSN - 2090-0201

DOI - 10.4061/2011/480570

Subject(s) - osmium , dna methylation , dna , methylation , combinatorial chemistry , microbiology and biotechnology , chemistry , computational biology , biology , biochemistry , gene , gene expression , ruthenium , catalysis

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.

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