Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India | Zendy

Satpal Singh Bisht | Zendy; Amrita Kumari Panda | Zendy

Open Access

Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India

Author(s) -

Satpal Singh Bisht,

Amrita Kumari Panda

Publication year - 2011

Publication title -

biotechnology research international

Language(s) - English

Resource type - Journals

eISSN - 2090-3138

pISSN - 2090-3146

DOI - 10.4061/2011/452710

Subject(s) - genbank , thermophile , 16s ribosomal rna , lipase , bacteria , accession number (library science) , hot spring , biology , ribosomal rna , strain (injury) , microbiology and biotechnology , acinetobacter , veterinary medicine , food science , gene , enzyme , biochemistry , medicine , genetics , paleontology , anatomy

Three lipase-producing thermophilic bacteria (AK-P1, AK-P2, and AK-P3) were isolated from the Taptapani hot water spring in Orissa, India. The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences submitted to NCBI GenBank. Strain AK-P3, exhibited the highest lipolytic activity of 5.5 U/mL was identified as Porphyrobacter sp. The lipolytic activities of strains AK-P1 and AK-P 2 were 4.5 U/mL and 3.5 U/mL, respectively. Strains AK-P1 and AK-P2 were identified as Acinetobacter sp. and Brevibacillus spp. The GenBank accession numbers of the 16S rRNA gene sequences determined in this study for the strains AK-P1, AK-P2, and AK-P3 are HM359120, HM359119, and HM359118, respectively

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