High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer′s Disease Biomarker Development | Zendy

Keiro Shirotani | Zendy; Satoshi Futakawa | Zendy; Kiyomitsu Nara | Zendy; Kyoka Hoshi | Zendy; Toshie Saito | Zendy; Yuriko Tohyama | Zendy; Shinobu Kitazume | Zendy; Tatsuhiko Yuasa | Zendy; Masakazu Miyajima | Zendy; Hajime Arai | Zendy; Atsushi Kuno | Zendy; Hisashi Narimatsu | Zendy; Yasuhiro Hashimoto | Zendy

Open Access

High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer′s Disease Biomarker Development

Author(s) -

Keiro Shirotani,

Satoshi Futakawa,

Kiyomitsu Nara,

Kyoka Hoshi,

Toshie Saito,

Yuriko Tohyama,

Shinobu Kitazume,

Tatsuhiko Yuasa,

Masakazu Miyajima,

Hajime Arai,

Atsushi Kuno,

Hisashi Narimatsu,

Yasuhiro Hashimoto

Publication year - 2011

Publication title -

international journal of alzheimer s disease

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.657

H-Index - 49

eISSN - 2090-8024

pISSN - 2090-0252

DOI - 10.4061/2011/352787

Subject(s) - transferrin , medicine , glycan , biomarker , cerebrospinal fluid , disease , measure (data warehouse) , extension (predicate logic) , alzheimer's disease , computational biology , bioinformatics , pathology , glycoprotein , computer science , biology , genetics , data mining , programming language

We have established high-throughput lectin-antibody ELISAs to measure different glycans on transferrin (Tf) in cerebrospinal fluid (CSF) using lectins and an anti-transferrin antibody (TfAb). Lectin blot and precipitation analysis of CSF revealed that PVL ( Psathyrella velutina lectin) bound an unique N-acetylglucosamine-terminated N-glycans on “CSF-type” Tf whereas SSA ( Sambucus sieboldiana agglutinin) bound α 2,6-N-acetylneuraminic acid-terminated N-glycans on “serum-type” Tf. PVL-TfAb ELISA of 0.5 μ L CSF samples detected “CSF-type” Tf but not “serum-type” Tf whereas SSA-TfAb ELISA detected “serum-type” Tf but not “CSF-type” Tf, demonstrating the specificity of the lectin-TfAb ELISAs. In idiopathic normal pressure hydrocephalus (iNPH), a senile dementia associated with ventriculomegaly, amounts of the SSA-reactive Tf were significantly higher than in non-iNPH patients, indicating that Tf glycan analysis by the high-throughput lectin-TfAb ELISAs could become practical diagnostic tools for iNPH. The lectin-antibody ELISAs of CSF proteins might be useful for diagnosis of the other neurological diseases.

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