Open AccessComparative Study of Serological Tests forMycoplasma synoviaeDiagnosis in Commercial Poultry Breeders
Author(s) -
Renato Luís Luciano,
A.L.S.P. Cardoso,
G. Stoppa,
Ana Maria Iba Kanashiro,
A.G.M. Castro,
E.N.C. Tessari
Publication year - 2011
Publication title -
veterinary medicine international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 30
eISSN - 2090-8113
pISSN - 2042-0048
DOI - 10.4061/2011/304349
Subject(s) - mcnemar's test , serology , direct agglutination test , veterinary medicine , mycoplasma synoviae , medicine , kappa , haemagglutination inhibition , hemagglutination assay , mycoplasma gallisepticum , mycoplasma , biology , immunology , antibody , microbiology and biotechnology , titer , mathematics , statistics , geometry
Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (=2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (≤.001), and Kappa coefficient showed a weak correlation between the two techniques (=0.25; 0.21≤<0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS
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