Open AccessAntibodies Induced by Lipoarabinomannan in Bovines: Characterization and Effects on the Interaction betweenMycobacterium aviumSubsp.paratuberculosisand MacrophagesIn Vitro
Author(s) -
Ana Jolly,
Silvia Beatriz Colavecchia,
Bárbara Fernández,
Eloy Fernández,
Silvia Leonor Mundo
Publication year - 2011
Publication title -
veterinary medicine international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 30
eISSN - 2090-8113
pISSN - 2042-0048
DOI - 10.4061/2011/258479
Subject(s) - lipoarabinomannan , paratuberculosis , antibody , mycobacterium bovis , microbiology and biotechnology , immunization , immune system , mycobacterium , antigen , isotype , immunology , in vitro , biology , medicine , mycobacterium tuberculosis , monoclonal antibody , tuberculosis , biochemistry , pathology
Lipoarabinomannan (LAM) is a major glycolipidic antigen on the mycobacterial envelope. The aim of this study was to characterize the humoral immune response induced by immunization with a LAM extract in bovines and to evaluate the role of the generated antibodies in the in vitro infection of macrophages with Mycobacterium avium subsp. paratuberculosis (MAP). Sera from fourteen calves immunized with LAM extract or PBS emulsified in Freund's Incomplete Adjuvant and from five paratuberculosis-infected bovines were studied. LAM-immunized calves developed specific antibodies with IgG1 as the predominant isotype. Serum immunoglobulins were isolated and their effect was examined in MAP ingestion and viability assays using a bovine macrophage cell line. Our results show that the antibodies generated by LAM immunization significantly increase MAP ingestion and reduce its intracellular viability, suggesting an active role in this model
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