Changes in Adenylate Nucleotides Concentration and , -ATPase Activities in Erythrocytes of Horses in Function of Breed and Sex

The aim of this study was to examine the relationships between the concentrations of ATP, ADP, AMP (HPLC methods), total nucleotide pool (TAN), adenylate energy charge (AEC) and Na + , K + -ATPase erythrocytic activities (by Choi's method) of horses as a function of breed and sex. The studies were conducted on 54 horses (stallions and mares) of different constitution types: breathing constitution (Wielkopolska and Hanoverian breed) and digestive constitution (Ardenian breed). Horse erythrocytes, independently of examined breed, present low ATP concentration in comparison to other mammal species while retaining relatively high AEC. Erythrocytes of breathing constitution type horses appear to have a more intensive glucose metabolism and a more efficient energetic metabolism when compared to digestive constitution type horses. The conclusions may be proven by significantly higher ATP concentration, higher TAN and significantly higher AEC in breathing constitution type horses compared to the digestive constitution type. Sex does not significantly influence adenine nucleotides concentration in the erythrocytes of the examined horses, however, stallions have slightly higher values in comparison to mares. A positive correlation was found between Na + , K + , -ATPase activity, ATP, ADP and AMP concentration and TAN in Wielkopolska and Ardenian breeds, which was not confirmed for the Hanoverian breed.