High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003
Author(s) -
Yingying Liu,
Weixing Ye,
Jinshui Zheng,
Lei Fang,
Donghai Peng,
Lifang Ruan,
Ming Sun
Publication year - 2014
Publication title -
standards in genomic sciences
Language(s) - English
Resource type - Journals
ISSN - 1944-3277
DOI - 10.4056/sigs.4738557
Subject(s) - bacillus thuringiensis , biology , plasmid , genome , gene , bacillus cereus , whole genome sequencing , genetics , microbiology and biotechnology , strain (injury) , chromosome , shotgun sequencing , bacteria , anatomy
Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. SDS-PAGE analysis of spore-crystal suspension of this strain reveals six major protein bands, which implies the presence of multiple parasporal crystal genes. Bioassay of this strain reveals that it shows specific activity against nematodes and human cancer cells. In this study, we report the whole genomic shotgun sequences of Sbt003. The high-quality draft of the genome is 6,175,670 bp long (including chromosome and plasmids) with 6,372 protein-coding and 80 RNA genes.
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