Borrelia miyamotoi Activates Human Dendritic Cells and Elicits T Cell Responses

The spirochete Borrelia miyamotoi has recently been shown to cause relapsing fever. Like the Lyme disease agent, Borrelia burgdorferi, B. miyamotoi is transmitted through the bite of infected ticks; however, little is known about the response of the immune system upon infection. Dendritic cells (DCs) play a central role in the early immune response agains B. burgdorferi We investigated the response of DCs to two different strains of B. miyamotoi using in vitro and ex vivo models and compared this to the response elicited by B. burgdorferi. Our findings show tha B. miyamotoi is phagocytosed by monocyte-derived DCs, causing upregulation of activation markers and production of proinflammatory cytokines in a similar manner to B. burgdorferi. Recognition of B. miyamotoi was demonstrated to be partially mediated by TLR2. DCs migrated out of human skin explants upon inoculation of the skin with B. miyamotoi. Finally, we showed tha B. miyamotoi -stimulated DCs induced proliferation of naive CD4 + and CD8 + T cells to a larger extent than B. burgdorferi. In conclusion, we show in this study that DCs respond to and mount an immune response agains B. miyamotoi hat is similar to the response to B. burgdorferi and is able to induce T cell proliferation.