Involvement of C-C Chemokine Ligand 2-CCR2 Interaction in Monocyte-Lineage Cell Recruitment of Normal Human Corneal Stroma | Zendy

Nobuyuki Ebihara | Zendy; Satoru Yamagami | Zendy; Seiichi Yokoo | Zendy; Shiro Amano | Zendy; Akira Murakami | Zendy

Open Access

Involvement of C-C Chemokine Ligand 2-CCR2 Interaction in Monocyte-Lineage Cell Recruitment of Normal Human Corneal Stroma

Author(s) -

Nobuyuki Ebihara,

Satoru Yamagami,

Seiichi Yokoo,

Shiro Amano,

Akira Murakami

Publication year - 2007

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.178.5.3288

Subject(s) - stromal cell , stroma , ccr2 , chemokine , ccl2 , monocyte , cornea , chemotaxis , cxcl1 , interleukin 8 , biology , microbiology and biotechnology , cxcl2 , immunology , chemistry , cancer research , cytokine , receptor , chemokine receptor , inflammation , immunohistochemistry , biochemistry , neuroscience

Bone marrow-derived cells (BMCs) reside in the anterior stroma of the central and paracentral cornea, as well as all stromal layers of the peripheral cornea, in normal human eyes. We investigated the factors regulating the constitutive distribution of BMCs in normal human corneal stroma. Cultured human corneal keratocytes expressed several chemokines (growth-related oncogene/CXCL1-3, IL-8/CXCL8, and MCP-1/CCL2) in the Ab array study. CCR2 and CCR7 mRNAs were detected in BMCs by multiplex RT-PCR. Keratocytes/corneal epithelial cells and BMCs selected from normal human donor corneas by using magnetic beads expressed MCP-1/CCL2 and CCR2 protein, respectively. BMCs isolated from human corneal stroma showed a chemotactic response to MCP-1/CCL2 in the Boyden chamber assay. The chemotactic effect of keratocyte supernatant was inhibited by blockade of MCP-1/CCL2. This is the first work on constitutive expression of CCR2 by BMCs from the corneal stroma and MCP-1/CCL2 by keratocytes/epithelial cells. Our findings suggest that the interaction between MCP-1/CCL2 and CCR2 determines the distribution of constitutive BMCs in normal human corneal stroma.

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