Open AccessCD8αα-Mediated Intraepithelial Lymphocyte Snatching of Thymic Leukemia MHC Class Ib Molecules In Vitro and In Vivo
Author(s) -
Nathalie Pardigon,
Kazuyo Takeda,
Bertrand Saunier,
Felicita Hornung,
James S. Gibbs,
Andrea S. Weisberg,
Nikhat Contractor,
Brian L. Kelsall,
Jack R. Bennink,
Jonathan W. Yewdell
Publication year - 2006
Publication title -
the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.177.3.1590
Subject(s) - intraepithelial lymphocyte , mhc class i , cd8 , biology , in vivo , jurkat cells , microbiology and biotechnology , major histocompatibility complex , phosphatidylinositol , in vitro , leukemia , mhc class ii , t cell , immunology , antigen , kinase , immune system , biochemistry , genetics
Thymic leukemia (TL) is a MHC class Ib molecule that interacts with CD8alphaalpha homodimers. CD8alphaalpha is abundantly expressed by intraepithelial T lymphocytes (IELs) located in close proximity to TL-expressing intestinal epithelial cells. In this study, we show that CD8alphaalpha(+) IELs "snatch" TL from the plasma membrane of TL-expressing cells and express TL in its proper orientation on their own cell surface. TL snatching is enhanced by cross-linking of IEL TCRs in a phosphatidylinositol kinase-dependent manner, and results in overall alterations to the IEL cell surface detected by enhanced binding of peanut agglutinin lectin. Induction of bowel inflammation results in the presence of TL on IELs, probably via in vivo snatching, providing the initial evidence for the interaction of CD8alphaalpha IELs with intestinal cells.
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