Cutting Edge: MyD88 Controls Phagocyte NADPH Oxidase Function and Killing of Gram-Negative Bacteria | Zendy

F. Stephen Laroux | Zendy; Xavier Romero | Zendy; Lee M. Wetzler | Zendy; Pablo Engel | Zendy; Cox Terhorst | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cutting Edge: MyD88 Controls Phagocyte NADPH Oxidase Function and Killing of Gram-Negative Bacteria

Author(s) -

F. Stephen Laroux,

Xavier Romero,

Lee M. Wetzler,

Pablo Engel,

Cox Terhorst

Publication year - 2005

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.175.9.5596

Subject(s) - nadph oxidase , phagocyte , superoxide , innate immune system , oxidase test , microbiology and biotechnology , gram negative bacteria , biology , bacteria , escherichia coli , immune system , biochemistry , reactive oxygen species , phagocytosis , enzyme , immunology , genetics , gene

MyD88 is an adaptor protein for the TLR family of proteins that has been implicated as a critical mediator of innate immune responses to pathogen detection. In this study, we report that MyD88 plays a crucial role in killing Gram-negative bacteria by primary macrophages via influencing NADPH oxidase function. Peritoneal macrophages from MyD88-/- mice exhibited a marked inability to kill Escherichia coli (F18) or an attenuated strain of Salmonella typhimurium (sseB) in vitro. This defect in killing was due to diminished NADPH oxidase-mediated production of superoxide anion in response to bacteria by MyD88-/- phagocytes as a consequence of defective NADPH oxidase assembly. Defective oxidase assembly in MyD88-deficient macrophages resulted from impaired p38 MAPK activation and subsequent phosphorylation of p47phox. Together these data demonstrate a pivotal role for MyD88 in killing Gram-negative bacteria via modulation of NADPH oxidase activity in phagocytic cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research