Open AccessB7-H3 Enhances Tumor Immunity In Vivo by Costimulating Rapid Clonal Expansion of Antigen-Specific CD8+ Cytolytic T Cells
Author(s) -
Liqun Luo,
Andrei I. Chapoval,
Dallas B. Flies,
Gefeng Zhu,
Fumiya Hirano,
Shengdian Wang,
Julie S. Lau,
Haidong Dong,
Koji Tamada,
Andrew S. Flies,
Yang Liu,
Lieping Chen
Publication year - 2004
Publication title -
the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.173.9.5445
Subject(s) - ctl* , cytolysis , biology , cd8 , cytotoxic t cell , in vivo , immune system , immunology , antigen , immunogenicity , t cell , microbiology and biotechnology , in vitro , cancer research , biochemistry
B7-H3 is a B7 family molecule with T cell costimulatory function in vitro. The in vivo role of B7-H3 in the stimulation of tumor immunity is unclear. We report here that expression of B7-H3 by transfection of the mouse P815 tumor line enhances its immunogenicity, leading to the regression of tumors and amplification of a tumor-specific CD8+ CTL response in syngeneic mice. Tumor cells engineered to express B7-H3 elicit a rapid clonal expansion of P1A tumor Ag-specific CD8+ CTL in lymphoid organs in vivo and acquire the ability to directly stimulate T cell growth, division, and development of cytolytic activity in vitro. Our results thus establish a role for B7-H3 in the costimulation of T cell immune responses in vivo.
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