Regulation of the Human FcεRI α-Chain Distal Promoter | Zendy

Masanari Hasegawa | Zendy; Chiharu Nishiyama | Zendy; Makoto Nishiyama | Zendy; Yushiro Akizawa | Zendy; Kyoko Takahashi | Zendy; Tomonobu Ito | Zendy; Susumu Furukawa | Zendy; Chisei Ra | Zendy; Ko Okumura | Zendy; Hideoki Ogawa | Zendy

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Regulation of the Human FcεRI α-Chain Distal Promoter

Author(s) -

Masanari Hasegawa,

Chiharu Nishiyama,

Makoto Nishiyama,

Yushiro Akizawa,

Kyoko Takahashi,

Tomonobu Ito,

Susumu Furukawa,

Chisei Ra,

Ko Okumura,

Hideoki Ogawa

Publication year - 2003

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.170.7.3732

Subject(s) - promoter , transcription (linguistics) , transcription factor , microbiology and biotechnology , response element , transcriptional regulation , biology , gene , reporter gene , promoter activity , gene expression , chemistry , genetics , linguistics , philosophy

The alpha-chain of the high affinity receptor for IgE (Fc epsilon RI) is essential for cell surface expression of Fc epsilon RI and binding of the IgE Ab. The human alpha-chain gene possesses two promoters: the proximal promoter, which is highly conserved with that of rodent; and the distal promoter, the structure and role of which are largely unknown. Transcriptional regulation of the alpha-chain distal promoter was investigated in this study. Transient reporter assay revealed critical region for transcription activity located within -27/-17. EMSA identified Elf-1, YY1, and PU.1 as transcription factors binding to this region. In contrast to the proximal promoter, which was trans-activated by YY1 and PU.1, these transcription factors exhibited repressive function on this promoter. Addition of IL-4 caused a marked increase in transcription from the distal promoter and subsequently increased the intracellular production of the alpha-chain. These results indicate that IL-4-dependent up-regulation of the human alpha-chain was due to enhancement of distal promoter activity and suggests that the two promoters have different regulatory mechanisms for alpha-chain expression.

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