Regulation of Cell Type-Specific Mouse FcεRI β-Chain Gene Expression by GATA-1 Via Four GATA Motifs in the Promoter | Zendy

Keiko Maeda | Zendy; Chiharu Nishiyama | Zendy; Tomoko Tokura | Zendy; Yushiro Akizawa | Zendy; Makoto Nishiyama | Zendy; Hideoki Ogawa | Zendy; Ko Okumura | Zendy; Chisei Ra | Zendy

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Regulation of Cell Type-Specific Mouse FcεRI β-Chain Gene Expression by GATA-1 Via Four GATA Motifs in the Promoter

Author(s) -

Keiko Maeda,

Chiharu Nishiyama,

Tomoko Tokura,

Yushiro Akizawa,

Makoto Nishiyama,

Hideoki Ogawa,

Ko Okumura,

Chisei Ra

Publication year - 2003

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.170.1.334

Subject(s) - microbiology and biotechnology , gene , biology , transcription factor , reporter gene , transcription (linguistics) , mast cell , promoter , cell , transcriptional regulation , regulatory sequence , gata transcription factor , gata2 , regulation of gene expression , gene expression , genetics , immunology , linguistics , philosophy

The FcR beta-chain, a subunit of two related multisubunit receptor complexes, the FcepsilonRI and FcgammaRIII, amplifies the mast cell response and is necessary for the cell surface expression of FcepsilonRI in mouse. The transient reporter assay indicated that -69/+4 region is required for cell type-specific transcriptional regulation of mouse beta-chain gene. EMSA using Abs against transcription factors or competitive oligonucleotides demonstrated that -58/-40 region (containing overlapping three GATA-1 sites, -53/-48, -46/-51, and -42/-47) and -31/-26 region (containing one GATA-1 site) are recognized by GATA-1. The promoter activity of beta-chain was decreased by nucleotide replacements of the GATA-1 sites in mouse mast cell line PT18. Furthermore, exogenously produced GATA-1 up-regulated the promoter activity in CV-1 cells, which are negative in the beta-chain production and the up-regulation was apparently suppressed by GATA-1 site mutations. These results indicate that cell type-specific transcription of mouse beta-chain gene is regulated by GATA-1.

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