Cutting Edge: TCR Engagement and Triggering in the Absence of Large-Scale Molecular Segregation at the T Cell-APC Contact Site | Zendy

Rossana Zaru | Zendy; Thomas O. Cameron | Zendy; Lawrence J. Stern | Zendy; Sabina Müller | Zendy; Salvatore Valitutti | Zendy

Open Access

Cutting Edge: TCR Engagement and Triggering in the Absence of Large-Scale Molecular Segregation at the T Cell-APC Contact Site

Author(s) -

Rossana Zaru,

Thomas O. Cameron,

Lawrence J. Stern,

Sabina Müller,

Salvatore Valitutti

Publication year - 2002

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.168.9.4287

Subject(s) - t cell receptor , immunological synapse , intracellular , microbiology and biotechnology , t cell , jurkat cells , activator (genetics) , signal transduction , biology , chemistry , biophysics , receptor , immunology , biochemistry , immune system

We investigated the functional role of large-scale molecular segregation at the T cell-APC contact site during T lymphocyte Ag recognition. Inhibition of CD2-CD58 interaction markedly affected segregation of CD2 and CD2AP from CD45. Under these conditions, Ag-induced calcium mobilization, PKC theta; clustering at the immunological synapse, and IFN-gamma production also were inhibited. However, early TCR signaling and T cell polarization toward APCs were unaffected. Our results indicate that the "raison d'être" of a large-scale segregation of surface molecules and intracellular enzymes and adapters, in Ag-stimulated T cells, is to reinforce the assembly of the signal transduction cascade rather than favor TCR engagement and triggering.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research