A Distinct Pathway of Cell-Mediated Apoptosis Initiated by Granulysin | Zendy

Allan Kaspar | Zendy; Satoshi Okada | Zendy; Jayant Kumar | Zendy; Francis R. Poulain | Zendy; Katerina A. Drouvalakis | Zendy; Ameeta Kelekar | Zendy; Dennis A. Hanson | Zendy; Ruth M. Kluck | Zendy; Yasumichi Hitoshi | Zendy; Daniel E. Johnson | Zendy; Christopher J. Froelich | Zendy; Craig B. Thompson | Zendy; Donald D. Newmeyer | Zendy; Alberto Anel | Zendy; Carol Clayberger | Zendy; Alan M. Krensky | Zendy

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A Distinct Pathway of Cell-Mediated Apoptosis Initiated by Granulysin

Author(s) -

Allan Kaspar,

Satoshi Okada,

Jayant Kumar,

Francis R. Poulain,

Katerina A. Drouvalakis,

Ameeta Kelekar,

Dennis A. Hanson,

Ruth M. Kluck,

Yasumichi Hitoshi,

Daniel E. Johnson,

Christopher J. Froelich,

Craig B. Thompson,

Donald D. Newmeyer,

Alberto Anel,

Carol Clayberger,

Alan M. Krensky

Publication year - 2001

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.167.1.350

Subject(s) - granulysin , granzyme , cytochrome c , ctl* , microbiology and biotechnology , apoptosis , granzyme b , programmed cell death , cytotoxic t cell , caspase , biology , mitochondrion , fas ligand , perforin , chemistry , biochemistry , in vitro

Granulysin is an antimicrobial and tumoricidal molecule expressed in granules of CTL and NK cells. In this study, we show that granulysin damages cell membranes based upon negative charge, disrupts the transmembrane potential (Deltapsi) in mitochondria, and causes release of cytochrome c. Granulysin-induced apoptosis is blocked in cells overexpressing Bcl-2. Despite the release of cytochrome c, procaspase 9 is not processed. Nevertheless, activation of caspase 3 is observed in granulysin-treated cells, suggesting that granulysin activates a novel pathway of CTL- and NK cell-mediated death distinct from granzyme- and death receptor-induced apoptosis.

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