Open AccessImproving Vaccine Potency Through Intercellular Spreading and Enhanced MHC Class I Presentation of Antigen
Author(s) -
Chien-Fu Hung,
WenFang Cheng,
CheeYin Chai,
Keng-Fu Hsu,
Liangmei He,
Morris Ling,
T. C. Wu
Publication year - 2001
Publication title -
the journal of immunology/the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.166.9.5733
Subject(s) - potency , mhc class i , dna vaccination , antigen , intracellular , virology , in vivo , biology , cd8 , microbiology and biotechnology , immunology , in vitro , biochemistry , genetics , immunization
The potency of naked DNA vaccines is limited by their inability to amplify and spread in vivo. VP22, a HSV-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. Therefore, we created a novel fusion of VP22 with a model Ag, human papillomavirus type 16 E7, in a DNA vaccine that generated enhanced spreading and MHC class I presentation of AG: These properties led to a dramatic increase in the number of E7-specific CD8(+) T cell precursors in vaccinated mice (around 50-fold) and converted a less effective DNA vaccine into one with significant potency against E7-expressing tumors. In comparison, nonspreading VP22(1-267) mutants failed to enhance vaccine potency. Our data indicated that the potency of DNA vaccines may be dramatically improved through intercellular spreading and enhanced MHC class I presentation of Ag.