Role of Stat3 in Lipopolysaccharide-Induced IL-10 Gene Expression | Zendy

Elke Benkhart | Zendy; Maciej Siedlar | Zendy; Angela Wedel | Zendy; Thomas Werner | Zendy; HW Ziegler-Heitbrock | Zendy

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Role of Stat3 in Lipopolysaccharide-Induced IL-10 Gene Expression

Author(s) -

Elke Benkhart,

Maciej Siedlar,

Angela Wedel,

Thomas Werner,

HW Ziegler-Heitbrock

Publication year - 2000

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.165.3.1612

Subject(s) - stat3 , gene , transcription (linguistics) , promoter , promoter activity , microbiology and biotechnology , stat , transcription factor , gene expression , biology , sequence motif , cytokine , genetics , linguistics , philosophy

IL-10 is a unique cytokine because it is anti-inflammatory and immunosuppressive. IL-10 is regulated at the level of transcription, but the critical motifs and the relevant transcription factors controlling this gene have remained elusive to date. We now report that a sequence at -120 bp in the human IL-10 promoter binds Stat3 but no other Stat proteins. Mutation of this motif abrogates LPS-induced trans-activation. Overexpression of dominant negative Stat3 suppresses promoter activity, while wild-type Stat3 leads to an enhancement of this activity. Our results show that Stat3, by binding to a single motif in the IL-10 promoter, is controlling expression of the human IL-10 gene.

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