A Putative 12 Transmembrane Domain Cotransporter Expressed in Thymic Cortical Epithelial Cells | Zendy

Moon Gyo Kim | Zendy; Francis A. Flomerfelt | Zendy; Kee-Nyung Lee | Zendy; Chuan Chen | Zendy; Ronald H. Schwartz | Zendy

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A Putative 12 Transmembrane Domain Cotransporter Expressed in Thymic Cortical Epithelial Cells

Author(s) -

Moon Gyo Kim,

Francis A. Flomerfelt,

Kee-Nyung Lee,

Chuan Chen,

Ronald H. Schwartz

Publication year - 2000

Publication title -

the journal of immunology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.164.6.3185

Subject(s) - biology , stromal cell , microbiology and biotechnology , transmembrane protein , complementary dna , transmembrane domain , suppression subtractive hybridization , cdna library , gene , receptor , cancer research , biochemistry

We have isolated a full-length cDNA clone (thymic stromal origin (TSO)-1C12) from a SCID thymus library using a probe from a PCR-based subtractive library enriched for sequences from fetal thymic stromal cells. TSO-1C12 mRNA is expressed mainly in the thymic cortex and is highly enriched in SCID thymus. Expression per cell is highest during fetal thymus development and decreases after day 16. Antipeptide Abs immunoprecipitated a hydrophobic, plasma membrane glycoprotein (thymic stromal cotransporter, TSCOT) whose translated sequence has weak homology to bacterial antiporters and mammalian cation cotransporters with 12 transmembrane domains. TSCOT represents a new member of this superfamily that is highly expressed in thymic cortical epithelial cells.

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