Role of Activator Protein-1 in TCR-Mediated Regulation of the Murine fasl Promoter | Zendy

Ken Matsui | Zendy; Sheng Xiao | Zendy; Alan Fine | Zendy; ShyrTe Ju | Zendy

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Role of Activator Protein-1 in TCR-Mediated Regulation of the Murine fasl Promoter

Author(s) -

Ken Matsui,

Sheng Xiao,

Alan Fine,

ShyrTe Ju

Publication year - 2000

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.164.6.3002

Subject(s) - activator (genetics) , microbiology and biotechnology , promoter , transcription factor , fas ligand , biology , t cell receptor , footprinting , transcription (linguistics) , transfection , binding site , gene , t cell , genetics , gene expression , apoptosis , programmed cell death , linguistics , philosophy , immune system

The present study demonstrates that transcription factor interactions are important in regulating the murine fasl promoter following TCR-mediated activation. We used DNase I-footprinting, EMSAs, and transient transfection assays to identify the minimal TCR signal-responsive region within the fasl promoter. This region contains the previously identified binding sites for NF-kappaB and Egr and the AP-1 site identified in this study. We found that TCR signaling induces AP-1 binding to this site and regulates the fasl promoter function in a fashion dependent on NF-kappaB binding. However, mutation in the AP-1 site alone did not show a significant effect on the promoter function. The data suggest that the minimal promoter required at least two transcription factors to function.

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