Open AccessDevelopment and Analysis of Various Clonal Alloantigen- Dependent Cytotoxic Cell Lines from Channel Catfish
Author(s) -
Tor B. Stuge,
Melanie R. Wilson,
He Zhou,
Katherine S. Barker,
Eva Bengtén,
Greg Chinchar,
Norman W. Miller,
L. William Clem
Publication year - 2000
Publication title -
the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.164.6.2971
Subject(s) - cytotoxic t cell , catfish , biology , t cell receptor , clone (java method) , immunology , microbiology and biotechnology , t cell , gene , in vitro , genetics , immune system , fish <actinopterygii> , fishery
To determine the phenotypes of cytotoxic cells in channel catfish, clonal alloantigen-dependent leukocyte lines were established from mixed leukocyte cultures. Each clone was analyzed for expression of TCR alpha and beta genes by RT-PCR and for target cell specificity by 51Cr-release assay. Based on the above criteria, the following five different cell types were identified among the 19 clones analyzed: 1) TCR alphabeta+ allospecific cytotoxic cells, 2) TCR alphabeta+ nonspecific cytotoxic cells, 3) allospecific TCR alphabeta+ noncytotoxic cells, 4) TCR alphabeta- nonspecific cytotoxic cells, and 5) TCR alphabeta- allospecific cytotoxic cells. The demonstration of cloned, TCR alphabeta+, allospecific cytotoxic effectors provides the strongest evidence to date for the existence of cytotoxic T cells in fish.
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