Open AccessTransgenic Expression of Cyclin D1 in Thymic Epithelial Precursors Promotes Epithelial and T Cell Development
Author(s) -
David B. Klug,
Elizabeth Crouch,
Carla Carter,
Lezlee Coghlan,
Claudio J. Conti,
Ellen R. Richie
Publication year - 2000
Publication title -
the journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.737
H-Index - 372
eISSN - 1550-6606
pISSN - 0022-1767
DOI - 10.4049/jimmunol.164.4.1881
Subject(s) - cyclin d1 , tec , transgene , biology , cyclin d , cyclin , microbiology and biotechnology , genetically modified mouse , cyclin d2 , thymocyte , keratin , cyclin b , population , cancer research , t cell , immunology , cell cycle , cell , gene , genetics , immune system , ionosphere , physics , astronomy , demography , sociology
We previously reported that precursors within the keratin (K) 8+5+ thymic epithelial cell (TEC) subset generate the major cortical K8+5- TEC population in a process dependent on T lineage commitment. This report demonstrates that expression of a cyclin D1 transgene in K8+5+ TECs expands this subset and promotes TEC and thymocyte development. Cyclin D1 transgene expression is not sufficient to induce TEC differentiation in the absence of T lineage-committed thymocytes because TECs from both hCD3epsilon transgenic and hCD3epsilon/cyclin D1 double transgenic mice remain blocked at the K8+5+ maturation stage. However, enforced cyclin D1 expression does expand the developmental window during which K8+5+ cells can differentiate in response to normal hemopoietic precursors. Thus, enhancement of thymic function may be achieved by manipulating the growth and/or survival of TEC precursors within the K8+5+ subset.
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