GATA1-Deficient Dendritic Cells Display Impaired CCL21-Dependent Migration toward Lymph Nodes Due to Reduced Levels of Polysialic Acid | Zendy

Maaike R. Scheenstra | Zendy; Iris M. De Cuyper | Zendy; Filipe BrancoMadeira | Zendy; Pieter De Bleser | Zendy; Mirjam Kool | Zendy; Marjolein Meinders | Zendy; Mark Hoogenboezem | Zendy; Erik Mul | Zendy; Monika C. Wolkers | Zendy; Fiamma Salerno | Zendy; Benjamin Nota | Zendy; Yvan Saeys | Zendy; Sjoerd Klarenbeek | Zendy; Wilfred F. J. van IJcken | Zendy; Hamida Hammad | Zendy; Sjaak Philipsen | Zendy; Timo K. van den Berg | Zendy; Taco W. Kuijpers | Zendy; Bart N. Lambrecht | Zendy; Laura Gutiérrez | Zendy

Open Access

GATA1-Deficient Dendritic Cells Display Impaired CCL21-Dependent Migration toward Lymph Nodes Due to Reduced Levels of Polysialic Acid

Author(s) -

Maaike R. Scheenstra,

Iris M. De Cuyper,

Filipe BrancoMadeira,

Pieter De Bleser,

Mirjam Kool,

Marjolein Meinders,

Mark Hoogenboezem,

Erik Mul,

Monika C. Wolkers,

Fiamma Salerno,

Benjamin Nota,

Yvan Saeys,

Sjoerd Klarenbeek,

Wilfred F. J. van IJcken,

Hamida Hammad,

Sjaak Philipsen,

Timo K. van den Berg,

Taco W. Kuijpers,

Bart N. Lambrecht,

Laura Gutiérrez

Publication year - 2016

Publication title -

the journal of immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.737

H-Index - 372

eISSN - 1550-6606

pISSN - 0022-1767

DOI - 10.4049/jimmunol.1600103

Subject(s) - gata1 , ccl19 , dendritic cell , ccl21 , microbiology and biotechnology , polysialic acid , immune system , biology , cell migration , immunology , chemistry , haematopoiesis , chemokine , cell , cell adhesion , stem cell , neural cell adhesion molecule , genetics , chemokine receptor

Dendritic cells (DCs) play a pivotal role in the regulation of the immune response. DC development and activation is finely orchestrated through transcriptional programs. GATA1 transcription factor is required for murine DC development, and data suggest that it might be involved in the fine-tuning of the life span and function of activated DCs. We generated DC-specific Gata1 knockout mice (Gata1-KO DC ), which presented a 20% reduction of splenic DCs, partially explained by enhanced apoptosis. RNA sequencing analysis revealed a number of deregulated genes involved in cell survival, migration, and function. DC migration toward peripheral lymph nodes was impaired in Gata1-KO DC mice. Migration assays performed in vitro showed that this defect was selective for CCL21, but not CCL19. Interestingly, we show that Gata1-KO DC DCs have reduced polysialic acid levels on their surface, which is a known determinant for the proper migration of DCs toward CCL21.

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