Molecular diversity analysis of conserved Capsicum chinense Jacq. germplasm in Sri Lanka | Zendy

N. H. L. D. L. D. Nanayakkara | Zendy; S. K. Wasala | Zendy; N. M. Ubeysekara | Zendy; K. G. C. N. Jayarathne | Zendy; I. P. Wickremasinghe | Zendy

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Molecular diversity analysis of conserved Capsicum chinense Jacq. germplasm in Sri Lanka

Author(s) -

N. H. L. D. L. D. Nanayakkara,

S. K. Wasala,

N. M. Ubeysekara,

K. G. C. N. Jayarathne,

I. P. Wickremasinghe

Publication year - 2018

Publication title -

tropical agricultural research

Language(s) - English

Resource type - Journals

ISSN - 1016-1422

DOI - 10.4038/tar.v29i2.8291

Subject(s) - germplasm , agriculture , tropical agriculture , diversity (politics) , crop , resource (disambiguation) , sri lanka , microbiology and biotechnology , biology , agroforestry , sustainable agriculture , geography , agricultural science , political science , agronomy , ecology , environmental planning , computer network , tanzania , law , computer science

Among the Capsicum species, Capsicum chinense has the highest pungency, the highest antioxidant activity and a wide genetic diversity. This study was conducted to evaluate the genetic diversity of C. chinense (Nai miris) germplasm conserved at the Plant Genetic Resources Centre, Gannoruwa, Sri Lanka. Twenty five C .chinense germplasm, two C. annuum, two C. frutescens and one C. baccatum were analysed using 27 Simple Sequence Repeat markers. Total number of amplified alleles was 108 which varied from 1 to 6 per locus. The mean polymorphism information content value was 0.46. Dendogram based on Nei’s genetic distances showed three main clusters where 88% of C. chinense germplasm was grouped into one cluster and C. annuum and C. frutescens were separated into two clusters. A high genetic diversity was observed within C. chinense cluster. The genetic diversity identified in this study will be useful for correct identification, conservation and breeding activities of Capsicum species.

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