Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for Leishmania donovani infections in Sri Lanka | Zendy

Hemal Kothalawala | Zendy; Nadira D. Karunaweera | Zendy

Open Access

Loop-mediated isothermal amplification assay as a sensitive diagnostic tool for Leishmania donovani infections in Sri Lanka

Author(s) -

Hemal Kothalawala,

Nadira D. Karunaweera

Publication year - 2016

Publication title -

ceylon medical journal

Language(s) - English

Resource type - Journals

eISSN - 2386-1274

pISSN - 0009-0875

DOI - 10.4038/cmj.v61i2.8286

Subject(s) - ceylon , medical journal , medicine , sri lanka , leishmania donovani , tropical medicine , family medicine , library science , pathology , leishmaniasis , visceral leishmaniasis , socioeconomics , ancient history , tanzania , sociology , computer science , history

Cutaneous leishmaniasis (CL) in Sri Lanka is caused by Leishmania donovani MON 37. Confirmation of diagnosis is done through microscopy, either directly or after in vitro culture. Molecular diagnostic methods are sensitive, but require well established laboratories. Loop mediated isothermal amplification assay (LAMP) is rapid, specific for parasite speciesspecific DNA amplification, and requires only basic laboratory equipment. The aim of the study was to determine the potential utility of LAMP to diagnose leishmaniasis.

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