Genotypes of hepatitis B virus identified in patients tested prior to endoscopy from a Teaching Hospital in the Central Province of Sri Lanka | Zendy

D.G.D. Jayasuriya | Zendy; F. Noordeen | Zendy; Fathima Nuzra Nagoor Pitchai | Zendy

Open Access

Genotypes of hepatitis B virus identified in patients tested prior to endoscopy from a Teaching Hospital in the Central Province of Sri Lanka

Author(s) -

D.G.D. Jayasuriya,

F. Noordeen,

Fathima Nuzra Nagoor Pitchai

Publication year - 2015

Publication title -

ceylon medical journal

Language(s) - English

Resource type - Journals

eISSN - 2386-1274

pISSN - 0009-0875

DOI - 10.4038/cmj.v60i2.8153

Subject(s) - medicine , genotype , hepatitis b virus , genotyping , nested polymerase chain reaction , virology , gene , hepatitis b , sri lanka , hepatitis c virus , virus , polymerase chain reaction , genetics , biology , history , ethnology , south asia

The present study was carried out to identify the hepatitis B virus (HBV) genotypes in six patients attending the surgical clinic who were positive for hepatitis B surface antigen. DNA was extracted from the serum of patients and subjected to a modified nested PCR to detect a 585 bp region within the S gene of the HBV genome. Positive PCR products were purified and sequenced via a cycle sequencing method. The sequence data were analyzed with reference sequences in the HepSEQ database to identify the particular HBV genotype of the samples. Nested PCR for the S gene of the HBV genome was positive in 2 out of 6 samples. The genotyping and sequence analysis of the PCR products showed HBV genotype A with a homology of 98% to the reference sequences in the HepSeq database.

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